1. Select appropriate multi-sample needle for required specimen. Remove the cover from the valve section of the needle.
2. Thread the needle Intathe holder. Be sure the needle Is firmly seated to avoid its removal during use.
3. Apply tourniquet. Prepare venipuncture site with an appropriate antiseptic. Do not palpate venipuncture area aftercieansing. Place patient's arm in a downward position     and Insert the needle into the vein as usual.
4. When penetrating thestopper,centertubes in holder to prevent sidewall penetration and resultant premature vacuum loss. Push tube into the holder and onto the needIe    valve puncturing the rubber stopper. Hold in place with the thumb, The prespeclfled vacuum of the tube allows the required quantity of blood to flow Into the tube,      Remove the tourniquet as soon as blood appears In the tube,
5. For proper additive performance, gently invert the tubes s-8 times immediately after collection. As soon as blood stops flowing in the last tube, remove needle from      vein, and apply pressure to puncture site with dry sterile swab until bleeding stops.
6. Dispose of needle and holder according to your facility's policies and guidelines,

• Note:Venipuncturetechnique is almost the same when blood collection set is used,But be sure you twist the luer adaptor deasil before puncture, preventing vacuum loss or blood leakage during collection, Venipuncture proceeds as normal and blood flashback Is clearly seen through the translucent shield.